Ciprofloxacin hydrochloride

概览
Description Ciprofloxacin hydrochloride is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity.
IC50 & Target Bacterial
In Vitro Ciprofloxacin hydrochloride is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity Ciprofloxacin hydrochloride (CIP) shows potent activity against Y. pestis with MIC90 of 0.03 μg/mL
In Vivo Ciprofloxacin hydrochloride (1 mg/L) induces glutathione-S-transferase (GST) activity, in contrast with inhibited GST and Catalase (CAT) of larvae exposed to enrofloxacin. Ciprofloxacin hydrochloride (≥10 μg/L) is ecotoxic for development, growth, detoxifying, and oxidative stress enzymes in anuran amphibian larvae[1]. In a murine model of pneumonic plague, Ciprofloxacin hydrochloride (30 mg/kg, i.p.) results in a drug exposure which is similar to the drug exposure observed in human following a 500 mg dose of oral Ciprofloxacin hydrochloride. Intraperitoneal Ciprofloxacin hydrochloride reduces the lung bacterial load compare to controls treated with intraperitoneal PBS
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg

 

1 mM 2.7189 mL 13.5943 mL 27.1887 mL
5 mM 0.5438 mL 2.7189 mL 5.4377 mL
10 mM 0.2719 mL 1.3594 mL 2.7189 mL

Please refer to the solubility information to select the appropriate solvent.

Cell Assay
Antibiotics (including Ciprofloxacin hydrochloride) are serially diluted 2-fold in 50 μL of cation-adjusted Mueller-Hinton broth (CAMHB). The antibiotic (including Ciprofloxacin hydrochloride) ranges are 8 to 0.004 μg/mL or 64 to 0.03 μg/mL based on a final well volume of 100 μL after inoculation.Bacterial inocula are prepared by suspending colonies into Mueller-Hinton broth (CAMHB) (containing Ciprofloxacin hydrochloride) from 18 to 24 h (B. anthracis) or 42 to 48 h (Y. pestis) on sheep blood agar (SBA) plates that are incubated at 35°C. Suspended cultures are diluted with CAMHB to a bacterial cell density of 105 CFU/mL adjusted based on the optical density at 600 nm. To each well of the 96-well plate, 50 μL of dilutions is added for a final inoculum of ~5×104 CFU/well. Plates are incubated at 35°C. MICs are determined visually at 18 to 24 h (B. anthracis) or 42 to 48 h (Y. pestis) and also by absorbance at 600 nm[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
 
Female BALB/cAnNCrl (BALB/c) mice, 8 to 10 weeks old and 20 g (±4 g) are used in this assay. A single dose of Ciprofloxacin hydrochloride (30 mg/kg) is administered to mice (n=30) via the intraperitoneal (i.p.) route. The mice (n=3/time point/group) are culled at 1, 10, 20, or 30 min and 1, 1.5, 2, 4, 8, 12 h following Ciprofloxacin hydrochloride administration and 1, 15, or 30 min and 1, 2, 4, 6, 10, 18, or 24 h following DRCFI or CFI administration. Blood sampling points are chosen based upon the short half-life of Ciprofloxacin hydrochloride and longer half-life of CFI. Blood and lungs (whole organ) are collected post mortem for analysis. The lung doses following CFI or DRCFI administration are calculated using the concentration of Ciprofloxacin hydrochloride in the lung samples at 1 min post-administration. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight 367.8
Formula C₁₇H₁₉ClFN₃O₃
CAS No. 93107-08-5
SMILES [H]Cl.O=C(C1=CN(C2CC2)C3=C(C=C(F)C(N4CCNCC4)=C3)C1=O)O
Storage 4°C, protect from light, stored under nitrogen*The compound is unstable in solutions, freshly prepared is recommended.
Shipping Room temperature in continental US; may vary elsewhere
Solvent & Solubility H2O: 12.5 mg/mL* “<1 mg/mL” means slightly soluble or insoluble. “≥” means soluble, but saturation unknown.
COA
Ciprofloxacin hydrochloride-COA HUISUN
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