概览
Description Colistin, a polypeptide antibiotic, is bactericidal to gram-negative bacteria by interfering with the structure and function of the outer and cytoplasmic membranes of bacteria.
In Vitro Colistins are bactericidal to gram-negative bacteria by a detergent-like mechanism. This mechanism involves interaction with lipopolysaccharides and phospholipids of the outer membrane and electrostatic interference with the outer membrane by competitively displacing divalent cations (calcium and magnesium) from the negatively charged phosphate groups of membrane lipids[1]. Colistin (polymyxin E) owns favorable properties of rapid bacterial killing, a narrow spectrum of activity, and an associated slow development of resistance for the treatment of infections caused by multidrug-resistant gram-negative bacteria. There are two forms of colistin available commercially: colistin (sulfate) mainly for topical use and colistin methanesulfonate (sodium) for parenteral use[2].
In Vivo High concentrations of colistin in rat ELF are achieved as a result of slow and sustained CMS conversion following i.t. instillation[3]. Colistin is often used in piglets but underdosing and overdosing are frequent. Under- or overdoses of colistin do not result in any major disturbance of piglet fecal microbiota and rarely select for chromosomal resistance in the dominant E. coli population[4].
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg

 

1 mM 0.7978 mL 3.9888 mL 7.9776 mL
5 mM 0.1596 mL 0.7978 mL 1.5955 mL
10 mM 0.0798 mL 0.3989 mL 0.7978 mL

Please refer to the solubility information to select the appropriate solvent.

Animal Administration
[3]
Rats: Colistin methanesulfonate (sodium) and colistin (sulfate) dosing solutions are freshly prepared in sterile 0.9% sodium chloride. For the i.v. studies, colistin methanesulfonate (CMS) or sulfate solutions are administered by a bolus injection via the jugular vein cannula. Intratracheal (i.t.) instillation is utilized as the technique for pulmonary administration. Animals are administered i.v. CMS at doses of 14 mg/kg of body weight, 28 mg/kg or 56 mg/kg. In an independent study, rats are administered i.v. colistin at doses of 0.21 mg/kg, 0.41 mg/kg, or 0.62 mg/kg[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight 1253.51
Formula C₅₂H₁₀₀N₁₆O₁₇S
CAS No. 1264-72-8
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month

 

Shipping Room temperature in continental US; may vary elsewhere
Solvent & Solubility H2O: ≥ 32 mg/mL* “<1 mg/mL” means slightly soluble or insoluble. “≥” means soluble, but saturation unknown.
COA
Colistin (sulfate)-COA HUISUN
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